General Cell Lines and Cell Cultures
Eurofins Discoverx cAMP Hunter CHO-K1 HTR1B Gi cell Line, 2 vials. (95-0162C2)
The cAMP Hunter HTR1B Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1B, a naturally Gi/Go coupled GPCR. Upon activation of HTR1B, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen MC3 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS022M)
The ChemiScreen MC3 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC3R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Stable Cell Line, 2 vials. (HTS010C)
The ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MIP, RANTES) based activation of CCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR5 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR5, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus macaque version of the protein. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen κ Opioid Receptor Stable Cell Line, 2 vials. (HTS095C)
The ChemiScreen κ Opioid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE, DPDPE) based activation of OPRK1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRK1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OPRK1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen VPAC1 Receptor Membrane Preparation, 1 vial. (HTS043M)
The ChemiScreen VPAC1 Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of VIPR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PrecisION hASIC3 Recombinant Stable Cell Line, 2 vials. (CYL3055)
The PrecisION hASIC3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying ASIC3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses ASIC3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PrecisION hKv2.1/hKv9.2 Recombinant Stable Cell Line, 2 vials. (CYL3067)
The PrecisION hKv2.1/hKv9.2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv2.1/Kv9.2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv2.1/Kv9.2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen MC5 Melanocortin Receptor Stable Cell Line, 2 vials. (HTS155C)
The ChemiScreen MC5 Melanocortin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MC5R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen V1B Vasopressin Receptor Membrane Preparation, 1 vial. (HTS136M)
The ChemiScreen V1B Vasopressin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AVPR1B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen CB1 Cannabinoid Receptor Membrane Preparation, 1 vial. (HTS019M)
The ChemiScreen CB1 Cannabinoid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CNR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx cAMP Hunter CHO-K1 EBI2 Gi Cell Line, 2 vials. (95-0172C2)
The cAMP Hunter EBI2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. hydroxy/dihydroxycholesterol) based activation of EBI2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type EBI2, a naturally Gi/Go coupled GPCR. Upon activation of EBI2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter 1321N1 CALCR-RAMP3 Gs Cell Line, 2 vials. (95-0166C6)
The cAMP Hunter CALCR-RAMP3 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCR, a naturally Gs coupled GPCR, alongside modulator protein RAMP3. Upon activation of CALCR-RAMP3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCR alongside the receptor modifying protein RAMP3, which increases activity of the CALCR receptor For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 GABBR1-GABBR2 Gi Cell Line, 2 vials. (95-0165C2)
The cAMP Hunter GABBR1-GABBR2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GABA) based activation of GABBR1/GABBR2 GPCR heterodimer activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GABBR1, a naturally Gi/Go coupled GPCR, alongside its partner subunit GABBR2. Upon activation of GABBR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Borrelia garinii; subsp. bavariensis; Strain PBi
Borrelia garinii subsp. bavariensis strain PBi is a whole-genome sequenced bacterial type strain that was isolated from cerebrospinal fluid. This strain does not grow on agar.